In this study, we employed various biological approaches to identify and unveil the mechanisms by which the LCK-14-3-3ζ-TRPM8 axis regulates TRPM8 assembly, channel function, and LCK activity and highlighted the importance of TRPM8 phosphotyrosine at Y1022 on the pancreatic cancer cells, which may be a potential therapeutic target for pancreatic cancer. The gene discussed is TRPM8; the disease is pancreatic neoplasm.