One gene, Glyma.01G068000, encoding HSP90 was detected with downregulation in CK-SCN4, but two other genes, Glyma.09G131500 (hsp83) and Glyma.16G178800 (hsp83), were negatively regulated with significance in CK-SCN5, suggesting that PTI was triggered initially by both nematode infections and that later, ETI was activated by the SCN4 infection. This evidence concerns the gene VPS45 and infection.