In total, 595 non-hypopharyngeal and 67 hypopharyngeal cancers were compared using maftools. We did not find any significant differences (Figures 3A, B and Supplementary Data 2.3) in mutation frequency except for CASP8 mutations, which were very rare (p = 0.0058, OR 0.12, CI 0.0028–0.69) and HRAS mutation, which were absent (p = 0.025, OR = 0, CI 0–0.87) in hypopharyngeal cancers. This evidence concerns the gene CASP8 and hypopharynx cancer.