As shown in Figure 3G, the mutational frequencies (metastatic vs. primary) of CDKN2B (14.8% vs. 0%, p = 0.001), FAT3 (7.4% vs. 0%, p = 0.041), MTAP (13% vs. 1.6%, p = 0.023), and SMAD4 (31.4% vs. 15.6%, p = 0.049) in metastatic tumors were significantly higher than in primary tumors. Here, SMAD4 is linked to metastatic neoplasm.