Both SKOV3 and ES-2 subcutaneous metastatic tumors were clearly visualized by 68Ga-NGR-RGD PET imaging at 1 h post tracer injection, although SKOV3 cells expressed a high level of integrin αvβ3 but relatively low level of CD13, and ES-2 expressed a high level of CD13 but low integrin αvβ3. Here, ANPEP is linked to metastatic neoplasm.