In this study, we validated a multiplex immunofluorescence panel of immune cell markers CD8, CD68, CD16, the immune checkpoint PD-L1, and melanoma tumour marker SOX10 with single-plex immunofluorescence staining using the Opal workflow along with the golden standard traditional chromogenic immunohistochemistry in a melanoma tissue microarray. This evidence concerns the gene CD8A and neoplasm.