Finally, to deeply investigate how the NAP1L1 promotes HCC progression, the HCC patients were separated into the high and low expression groups ground on the expression of NAP1L1. Furthermore, we took multiple GSEA employing the GO and the KEGG, and the enrichment results of the top five genes of upregulation and the top five genes of downregulation were displayed in GO and KEGG gene sets (Figures 4H,I, Supplementary Figures S1H, S1I, S2H, S2I, S3H, S3I). This evidence concerns the gene NAP1L1 and hepatocellular carcinoma.