SDPL1 expression level was associated with lymph node metastasis (N0-vs-N1; N0-vs-N2; N0-vs-N3), TP53 mutation (TP53-mutant-vs-TP53-non-mutant), age ((age (41–60yrs)-vs-age (61–80yrs)) and (age (41–60yrs)-vs-age (81–100yrs)), grade (grade2-vs-grade3), drinking history (0 Ddys/week-vs-7 Ddays/week, 1 day/week-vs-4days/week, 4 ay/week-vs-5 days/week, 4 day/week-vs-7 days/week, and 5 day/week-vs-7 days/week), and cancer stage (stage1-vs-stage2, stage1-vs-stage3, and stage1-vs-stage4) of ESCA patients which was significant. The gene discussed is TP53; the disease is metastatic malignant neoplasm in the lymph nodes.