NOS2 and neoplasm: However, although an increase in pro-inflammatory macrophages has been reported previously both in full-body and myeloid-specific Trib1 knockout animals 19, 21, inhibition of myeloid Trib1 expression did not alter the ratio of NOS2+ pro-inflammatory TAMs and mannose receptor (MR)+, anti-inflammatory TAMs in the TME (Figure 3D, Supplementary Figure 2F, Supplementary figure 3), suggesting that the reduced TAM numbers, rather than their altered inflammatory phenotypes, may have contributed to the accelerated tumor growth.