TRIB1 and neoplasm: In order to gain a mechanistic insight into how TRIB1 regulates monocyte-derived macrophages and the impact of reduced TRIB1 expression on re-educating macrophages towards TAMs, human MDMs were transfected with siRNA against TRIB1 (MTRIB1-KD), followed by a treatment with tumor-conditioned medium (CM) (TAMTRIB1-KD) which resulted in ~50% reduction in TRIB1 expression (Figure 3M).