MAPK3 and infectious otitis media: To rule out any potential confounding effect of changes in development with Htr2b knockout, we generated mice containing floxed Htr2b alleles and the villin-Cre-ERT2 driver (hereafter referred to as Htr2bΔIEC-ER mice), which allows controlled gene ablation in IECs upon tamoxifen treatment; we administered tamoxifen 5 times during the first DSS treatment window in the AOM/DSS treatment regime (Figure S2C).