AKT1 and colon carcinoma: To further explore the role of Akt in 5HT signaling, we used the CRISPR-Cas9 technique to knock out Akt1 in CT26.WT cells, a mouse colon carcinoma cell line, and screened out two monoclonal cell lines: Akt1 KO1 and Akt1 KO2 (Figure S6A).