DAPK1 and glioblastoma: To further validate the role of UNC5B-DAPK1 pathway in PTBP1 knockdown and the resulting reprogramming of glioblastoma cells, subsequent experiments were conducted for blocking tests and six groups were created: sh-Luci, sh-PTBP1, sh-PTBP1 + vehicle, and sh-PTBP1 + varying concentrations of TC-DAPK 6 (100 nM, 250 nM, and 500 nM).