To further explore the effect of JHU083 on the differentiation of CD4(+) and CD8(+) T cells in AIH mice, the differentiation marker of CD4(+) (IFNγ for Th1 cells, IL4 for Th2 cells and IL17A for Th17 cells) and CD8(+) T cells (IFNγ and Granzyme B for CTLs) were evaluated using flow cytometry. This evidence concerns the gene CD4 and autoimmune hepatitis.