Other significant factors associated with BCR on univariate analysis included preoperative PSA levels (p=0.022), positive digital rectal exam (p=0.035), number of positive biopsy cores (p<0.001), unfavorable intermediate-risk group (p=0.014), perineural invasion on RP specimen (p=0.043), tumor volume (p=0.011), and positive surgical margins (p<0.001). This evidence concerns the gene KLK3 and neoplasm.