Upregulated TNF-α and MCP-1 caused renal tubular epithelial cells’ dysfunction, podocyte injury, mesangial cell proliferation and oxidative stress and induced mesangial cells, glomerulus and renal tubular epithelial cells to differentiate into fibroblasts, so as to enhance the production and deposition of ECM, which further enhances the progression of DN [32,33,34]. This evidence concerns the gene CCL2 and liver dysplastic nodule.