In addition, RT-PCR assay showed that the mRNA expression level of SGLT2 was also not upregulated in osteosarcoma patient specimens (Fig. 1b), while Western Blot analyses showed that the protein expression level of SGLT2 was significantly upregulated in osteosarcoma patient specimens (Fig. 1c). The gene discussed is SLC5A2; the disease is osteosarcoma.