In summary, the present study showed that treatment with compounds 4 or 6 significantly enhanced the biochemical parameters including liver enzymes, Vitamin-D level, CD4/CD8 ratio, and the level of antioxidant enzymes; while significantly decreasing the level of lipid peroxidation and pro-inflammatory cytokines in a dose-dependent manner in (NDEA + PB)-induced HCC rats. This evidence concerns the gene CD4 and hepatocellular carcinoma.