As such, sepsis was more severe in macrophage-depleted CLP mice than control CLP mice as indicated by survival analysis (Fig. 4A), liver injury (alanine transaminase) (Fig. 4B), renal damage (blood urea nitrogen (Fig. 4C), serum creatinine (Fig. 4D), kidney injury score and histology (H&E staining) (Fig. 4E, G), spleen apoptosis (activated caspase 3) and immunohistochemistry staining (Fig. 4F, H), serum cytokines (TNF-α, IL-6, and IL-10) (Fig. 4I–K) but not bacteremia and endotoxemia (Fig. 5A, B). Here, CASP3 is linked to serum lipopolysaccharide activity.