miR-195 is one of the key members of the micro-15/16/195/424/497 family, which can be activated in many diseases, such as cancer, hemangioma, and neuropathic pain.25, 26, 27 The mechanism of miR-195 mediated its action is extremely complex and involves various signal transduction pathways, such as Rb-E2F, PI3K/AKT, NF-kB, MAPK/REK, Wnt/ β -Catenin and so on.28, 29 This also confirms the findings of The present study’s KEGG pathway study. The gene discussed is RB1; the disease is cancer.