AIF1 and Huntington disease: Immunoblotting analyses of the microglial marker Iba1 (Ionized calcium binding adaptor molecule), considered a reactive marker of microgliosis, indicated an increase in total Iba1 protein levels between WT and the HD groups but immunohistological analyses of Iba1 showed no differences in the number or area size of Iba1+ cells across all genotypes (Additional file 10e-h), in line with the results obtained by RNA-seq (Fig. 4e).