We further performed supervised analysis to filter potential drug targets (https://www.proteinatlas.org/), and 23 genes (GARS, CFI, MYLK, etc.)met the criteria, in which six (GARS, CAV1, P4HA2, etc.)were reportedly correlated with poor prognosis and overrepresented in the staining of urothelial bladder cancer samples in the HPA database (Additional file 9: Fig. S9F–G; Additional file 19: Table S7). This evidence concerns the gene P4HA2 and bladder transitional cell carcinoma.