We identified 10 hub genes in IRI-AKI with one biomarker (Atf3) has been studied in IRI-AKI, five biomarkers (Cxcl1 and Jun, Fos, Nfkbiz, Hmox1) were researched in other types of AKI, 2 genes (Atf4 and Egr1) play role in I/R injury of other organs and three genes (Ppp1r15a, Hspa1b, and Ddit3) had not been reported in AKI or IRI researches. Here, PPP1R15A is linked to acute kidney injury.