The results showed that the expressions of GFAP and Iba-1 in the normal saline treatment group were much higher than those in the CsA@HFn, CsA and CsA@BSA treatment groups, indicating that cerebral ischemia reperfusion injury caused the recruitment of astrocyte and microglia to cerebral ischemic area in MCAO mice. Here, AIF1 is linked to brain ischemia.