1 d after adoptive T cell transfer, primary breast tumors from PyMttg TslprKO or MMTV-PyMT-mCherry-Ovatg, Tslpr−/− mice (PyMtOvatg TslprKO, expressing OVA in tumor cells; Engelhardt et al., 2012) on the C57BL/6 background were implanted into the abdominal mammary fat pad of recipient Tslptg TslprKO mice, which were then monitored for breast tumor growth over time (Fig. 5 A). The gene discussed is CRLF2; the disease is breast neoplasm.