ALK and non-small cell lung carcinoma: Furthermore, as observed in the ALK-positive NSCLC-derived cells, the HA-tagged V1 protein exhibited disperse cytoplasmic localization in metaphase Beas-2B cells with little association to microtubules (R = 0.27 ± 0.05), whereas HA-V3 exhibited strong spindle microtubule localization (R = 0.76 ± 0.03; Fig. 3G and H).