To evaluate the role played by PD-L1 in IFNγ signaling in ccRCC and to assess whether it might play a role in IFNγ-mediated regulation of cellular metabolism, we transiently silenced PD-L1 using a pooled small interference RNA on both A498 and 786-O cells, using scrambled RNA as controls. This evidence concerns the gene CD274 and nonpapillary renal cell carcinoma.