We also found that SETDB1 protein was mainly localized in the nucleoplasm of A-431 (human epithelial carcinoma cell line), U-2 OS (human osteosarcoma cells), and U-251 MG (human brain glioblastoma astrocytoma cancer cells) and vesicles of U-2 OS cell lines (Figure S1D). The gene discussed is SETDB1; the disease is osteosarcoma.