PRPH2 and central areolar choroidal dystrophy: The identification of genetic variants in individuals affected by CACD traditionally relied on chain termination sequencing of the three exons of the PRPH2 gene (Reig et al., 1995a; Hoyng et al., 1996b; Payne et al., 1998), often preceded by exploratory analyses using single-strand conformation polymorphism (Kohl et al., 1997b; Trujillo et al., 1998), denaturing gradient gel electrophoresis (Piguet et al., 1996; Payne et al., 1998) or heteroduplex analysis (Downes, 1999a).