Our results showed that RB treatment decreased the phospho-Src, FAK, and Paxillin levels, although it had no effect on focal adhesion-related total Src, FAK, and Paxillin levels (Figure 4C), suggesting that RB inhibited GBM cell invasion by suppressing the Src/FAK/Paxillin focal adhesion pathway. This evidence concerns the gene RB1 and glioblastoma.