The proliferation inhibition, cell cycle arrest, and apoptosis induction assays described above all conducted in these four drug-resistant pancreatic cancer cells, including BxPC3/MDR1, PANC1/MDR1, BxPC3/MRP1, and PANC1/MRP1, and these data of these four pancreatic cancer cell lines displayed basically consistent trends, which collectively indicated that GEM-HSA-NP could effectively inhibit proliferation, arrest cell cycle, and induce apoptosis of MDR1- and MRP1-overexpressing pancreatic cancer cells, thus reversing GEM resistance. Here, ABCB1 is linked to familial pancreatic carcinoma.