The cytokine IFN-γ and the cytolytic molecules granulysin and granzyme B were detected in supernatants of T-cell/tumor cell co-cultures using commercial ELISA kits (Human IFN gamma ELISA Ready-SET-Go!®, #88-7316-76, Affymetrix eBioscience; Human Granulysin DuoSet ELISA, #DY3138; and Human Granzyme B DuoSet ELISA, #DY2906-05, R&D Systems) in 96-well microtiter plates (Nunc Maxisorp, Rochester, NY, USA) following the manufacturer’s instructions. The gene discussed is GZMB; the disease is neoplasm.