To fully understand the consequences of the ESCCAL‐1‐miR‐590‐LRP6 interactions in ESCC tumorigenesis, ESCCAL‐1 was knocked down, or LRP6 (miR‐590) was over‐expressed in KYSE150 and KYSE450 cells. This evidence concerns the gene LRP6 and esophageal squamous cell carcinoma.