To fully understand the consequences of the ESCCAL‐1‐miR‐590‐LRP6 interactions in ESCC tumorigenesis, ESCCAL‐1 was knocked down, or LRP6 (miR‐590) was over‐expressed in KYSE150 and KYSE450 cells. The gene discussed is CASC9; the disease is esophageal squamous cell carcinoma.