Upregulation of miR-382-5p expression in hepatic cell lines (Hep3B and HCCLM3) showed an increase in the number of invaded cells in HCC, while after co-transfection of miR-382-5P and PTEN plasmids, the invasion ability of HCC cells was weakened, and the number of invaded cells was not significantly different from that of the scramble group. The gene discussed is PTEN; the disease is hepatocellular carcinoma.