Overall, high frequency of tumor-infiltrating FoxP3−Helios− T cells, and high frequencies of circulating FoxP3+, Helios+, FoxP3+Helios+, FoxP3+Helios−PD-1+, FoxP3+Helios+CTLA-4+, FoxP3+Helios−CTLA-4+ Tregs, and FoxP3−Helios+CTLA-4+ T cells, are associated with shorter DFS. The gene discussed is CTLA4; the disease is neoplasm.