In this study, we performed a prospective analysis to investigate whether extracellular vesicles isolated from plasma of patients with AIDS-NHL at baseline, and post-treatment, express molecules associated with B cell activation and immune signaling, such as PD-L1, CD40, CD40L, B7-H3, TNF-RII or IL-6Rα. This evidence concerns the gene TNFRSF1B and non-Hodgkin lymphoma.