Interestingly, our results showed that in three GBM cell lines (U-87 MG, A-172 and U-251 MG), lactate induced a significant increase in relative IGFBP6 mRNA expression levels following 24 h (U-87 MG: 10.48 ± 0.8; A-172: 12.79 ± 1.2; U-251 MG: 2.11 ± 0.16, Fig. 3A) and 48 h (U-87 MG: 19.16 ± 2.9; A-172: 4.96 ± 0.3; U-251 MG: 4.02± 0.3, Fig. 3A) of treatment compared to their untreated control cells. Here, IGFBP6 is linked to glioblastoma.