ATF4 and neoplasm: To delineate the role of host ATF4 in the sequence of events leading to tumour growth, we performed transcriptional profiling at the single-cell level (single cell RNA sequencing (scRNA-seq)) in small (150 mm3) and large (300 mm3) B16F10 tumours grown in Atf4WT/WT and Atf4Δ/Δ mice (Extended Data Fig. 2a).