In recent decades, most cancer studies have mainly focused on the variations in miRNA, RNA-binding proteins (RBPs), alternative polyadenylation (APA), and m6A RNA modification on the 3′-UTR of mRNA, but 3′-UTR mutations could also result in mRNA expression changes, and recurrent 3′-UTR mutations in cancer genes have been identified by whole-genome sequencing (WGS) to play vital roles in tumorigenesis2. The gene discussed is PTBP1; the disease is cancer.