First, Western diet feeding led to severe hypercholesterolemia with cholesterol levels over 1000 mg/dL in Apoe–/–Ndst1fl/flAlb-Cre+ and in Ldlr–/–Ndst1fl/flAlb-Cre+ mice and beyond 2000 mg/dL in Ldlr–/–Lrp1fl/flAlb-Cre+ mice. This evidence concerns the gene APOE and familial hypercholesterolemia.