To test the importance of HSP60 in a genetically engineered mouse model of PCa, we bred floxed Hsp60 alleles (54) into the TKO murine PCa model, which develops AR–, highly aggressive neuroendocrine PCa de novo due to concordant deletion of the Pten, Rb1, and p53 tumor suppressor genes in prostate epithelial cells (55). This evidence concerns the gene RB1 and posterior cortical atrophy.