To determine if lymphedema fluid can activate inflammatory responses in draining lymph nodes or local tissues, we harvested tissue lysate from the tails of animals that had tail surgery and were treated with either isotype control or TGF‐β1 neutralising antibodies beginning 2 weeks after surgery for 2 weeks (Figure 3F and Supplemental Figure S3E). The gene discussed is TGFB1; the disease is lymphedema.