We found the decreased expression levels of Alpha Fetoprotein (AFP), Cytokeratin 19 (CK19), and Aminopeptidase N (CD13) and an increased Cytokeratin 18 (CK18) expression in CU27‐treated cells compared with controls (Figure 2A,B; Figure S5A,B, Supporting Information), indicating a well differentiation status.[27] We have added CU27 and HCC cells together into the 3D tumorsphere system, and found that no tumorspheres were formed after 7‐days‐treatment of CU27 (Figure 2C; Figure S5C, Supporting Information). This evidence concerns the gene KRT18 and hepatocellular carcinoma.