PRF1 and non-small cell lung carcinoma: Interestingly, at days 1 and 3 of coculture the more invasive H1299 NSCLC induced hNK to produce significantly higher fold-increase in two key targets of Eomes (Araki et al., 2008), perforin (≈2.5 fold) and granzyme b (≈1.5 fold), compared to control hNK cells which were not challenged with NSCLCs (Figures 1C,D).