PRF1 and non-small cell lung carcinoma: Although both NSCLC subtypes induced Eomes nuclear localization in NK cells to different extents, we observed a particularly prominent nuclear localization when hNK cells were primed by the highly invasive metastatic NSCLC, H1299 (Figure 2), corresponding to up to 2.0-fold change in the production of perforin and granzyme b (Figures 1C,D).