Although the mROS and mitochondrial weight of CD4+ T cells treated with astilbin for 24 h showed no obvious changes, they decreased after 36-h treatment (Supplementary Figure S6), confirming the protective role of astilbin in cerebral ischemia/reperfusion injury by downregulating ROS-NLPR3 activation (Li et al., 2020). Here, CD4 is linked to Cerebral ischemia.