We found that MUC4 gene rs80145081, rs80005560 and rs142559357 are risk factors for GD in the Tibetan population of China, which is an effective supplement to the susceptibility site of MUC family gene in GD, and provides clues for exploring the mechanism of MUC family gene co-action to increase susceptibility to gallstones in Chinese population. The gene discussed is MUC4; the disease is gallstones.