Secondly, previous studies had shown that IL-1β, IL-6, and TNF-α are associated with ACS, but no association between these cytokines and the severity of CAD was found in the present study, and it was probably due to the exclusion of AMI patients and the lack of a large sample size, although the present study had a relatively large number of subjects, it was still inadequate compared to the large coronary heart disease population, the larger sample size was needed to make the findings generalizable. The gene discussed is TNF; the disease is coronary artery disorder.