As showed in the Figure 2, the analytic results revealed that the expression of four (HRAS, CDKN1A, TGFB1, H2AFX and ABL1) of eight targets of miR-663a was significantly upregulated in osteosarcoma tissue compared with normal tissue whereas TP53, PIK3CD and GRB2 were markedly downregulated. This evidence concerns the gene GRB2 and osteosarcoma.