Moreover, qRT-PCR experiments of the indicated cell lines showed that overexpression of RCN3 showed a significantly increased level of TGFβ1, IL-10, and IL-6, indicating that RCN3 might promote oncogenic progress of CRC with immunological regulation, which also matched our previous bioinfomatic analysis of its pan-cancer role (Figure 9D). This evidence concerns the gene TGFB1 and cancer.