When combined with antigen vaccines containing the melanoma TAAs gp100 or trp-1 and LPS or Poly-IC, IL-2/CD25 increased the frequency of effector and memory gp100-specific Pmel-1 CD8+ or TRP-1-specific TRP-1 CD4+ T cells post adoptive transfer (71, 102). The gene discussed is CD4; the disease is melanoma.