In addition, FOXM1 expression was enhanced by transfecting pcDNA3.1-FOXM1 in ESCC cells (Supplementary Figure S4A), and then the result of luciferase reporter assay manifested that HEK293T cells displayed an elevated luciferase activity in pGL3-HER2 promoter cotransfected with pcDNA3.1-FOXM1, while the corresponding mutant group exhibited no obvious change (Figure 3E). This evidence concerns the gene FOXM1 and esophageal squamous cell carcinoma.