In-depth mechanism research of PTBP1 revealed that PTBP1 was significantly correlated with the regulation of cell cycle, E2F targets, G2M checkpoint, MYC targets, DNA replication, ribosome, and spliceosome pathways in pan-cancer, suggesting that PTBP1 may play an important role in maintaining tumor cell viability to promote tumor proliferation. Here, PTBP1 is linked to neoplasm.